locus

BSU01530

BSU_01530

geneLength

711

714

outlinks

bsu

BSU01530

BSU_01530

The protein

Catalyzed reaction/ biological activity

formation of muramic acid delta-lacton in spore cortex peptidoglycan [Pubmed|14679227]

Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides (according to UniProt)

The protein

Protein family

N-acetylmuramoyl-L-alanine amidase 3 family (according to Swiss-Prot)

[SW|N-acetylmuramoyl-L-alanine amidase 3 family] (according to UniProt)

The protein

[SW|Domains]

contains an amidase_3 domain (like [[protein|CwlC]], [[protein|LytC]], [[protein|YqiI]], [[protein|YrvJ]])

[SW|MurNAc-LAA domain] (aa 43-226) (according to UniProt)

The protein

Paralogous protein(s)

[[this]]

The protein

Structure

[PDB|4RN7] (from Clostridium difficile, 30% identity, aa 43 - 234)

Biological materials

Mutant

BKE01530 (Δ[[gene|cwlD]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE01530 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCCCTTCCCCTCCCGCTT, downstream forward: _UP4_TAATGGAGGGTTTTCTTGTG

BKK01530 (Δ[[gene|cwlD]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK01530 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCCCTTCCCCTCCCGCTT, downstream forward: _UP4_TAATGGAGGGTTTTCTTGTG